Brijesh K. Dabhi, Kinnari N. Mistry, Jinal M. Thakor, Sishir Gang
Keywords
Diabetic nephropathy • Polymorphism • Cytokine expression • Genotype
Abstract
Objective Genetic polymorphisms of the angiogenesis, inflammatory cascade, or apoptosis genes can influence chronic complications in diabetic patients. Cytokine gene polymorphisms are considered vital in diabetes and diabetic nephropathy (DN) susceptibility. The present study evaluated the role of cytokine gene polymorphism in type 2 diabetes and diabetic nephropathy.
Methods A total number of 648 participants comprising 180 healthy individuals, 164 type 2 diabetes mellitus patients without any complications, 148 individuals with diabetic nephropathy, and 156 with non-diabetic nephropathy were included in this study. The IL-6 (-634 C/G), IL-18 (-607 A/C), IL-4 (-590C/T), and IL-10 (-592 C/A) polymorphism were analyzed using the PCR-RFLP method, and their expression analysis was done using real-time PCR
Results We found a significant difference in the genotype frequency of IL-6 and IL-10 in the diabetic nephropathy group compared to the control, whereas no significant difference was found in IL-18 and IL-4.
Conclusion The IL-6 -634C/G and IL-10-592 C/A polymorphisms were found to be associated with diabetic nephropathy in the West Indian population. The higher transcript level of inflammatory cytokines in patient groups compared to the control group may suggest the essential role of inflammation in the pathogenesis of diabetes and diabetic nephropathy
